Immunohematology Program Modules

Instructions: BB Specimens

(451 ABO & Rh Factor)

1. Remove the vials from the refrigerator. It is not necessary to warm the samples to room temperature.

2. The BB Specimens contain a 2-5% red cell suspension for ABO and Rh typing.

3. Before testing, mix contents of each vial by gently swirling. Be sure to re-suspend all red cells before proceeding.

4. Test each sample in the same manner as you would test a patient sample. If using a gel method, follow the gel card manufacturer’s instructions.

5. After sampling, replace the cap on each vial and return to the refrigerator.

 

Instructions: RH Specimens

(450 D (Rh) Typing)

The RH Specimens contain a 30% red cell suspension in normal saline.

1. Remove the vials from the refrigerator. Allow the samples to sit room temperature 10 – 15 minutes.

2. Before testing, mix contents of each vial by gently swirling. Be sure to re-suspend all red cells before proceeding.

3. Test each sample in the same manner as you would test a patient sample. If using a gel method, follow the gel card manufacturer’s instructions.

4. After sampling, replace the cap on each vial and return to the refrigerator.

Instructions: BB & AB Specimens

(452 Blood Bank 1)

1. Remove the vials from the refrigerator. It is not necessary to warm the samples to room temperature.

2. The BB Specimens contain a 2-5% red cell suspension for ABO and Rh typing.

3. The AB Specimens contain serum for ABO reverse typing and antibody detection/identification.

4. Before testing, mix contents of each vial by gently swirling. Be sure to re-suspend all red cells before proceeding.

5. Test each sample in the same manner as you would test a patient sample. If using a gel method, follow the gel card manufacturer’s instructions.

6. After sampling, replace the cap on each vial and return to the refrigerator.

 

Instructions: BB, AB & XM Specimens

(453 Blood Bank 2)

1. Remove the vials from the refrigerator. It is not necessary to warm the samples to room temperature.

2. The BB Specimens contain a 2-5% red cell suspension for ABO and Rh typing.

3. The AB Specimens contain serum for ABO reverse typing, antibody detection/antibody identification and compatibility testing.

4. The XM Specimen contains a 2-5% red cell suspension for crossmatching/compatibility testing with the serum AB specimens.

5. Before testing, mix contents of each vial by gently swirling. Be sure to re-suspend all red cells before proceeding.

6. Test each sample in the same manner as you would test a patient sample. If using a gel method, follow the gel card manufacturer’s instructions.

7. After sampling, replace the cap on each vial and return to the refrigerator.

Antibody Identification

• If the Antibody ID is not performed in-house, leave boxes blank.
• If the Antibody ID is performed in-house and Antibody Screen is negative, enter 010 Negative screen, ID not performed.
• Use 015 Would refer if unable to identify antibody with existing ID panel cells.
• Second Antibody ID is for documentation purposes only, if applicable. This not evaluated.

If the Compatibility Testing is not performed in‐house, leave the Compatibility box blank.

   

Instructions: DAT Specimens

(454 Direct Antiglobulin Test)

The DAT Specimens contain a 2-5% red cell suspension in normal saline.

1. Remove the vials from the refrigerator. Allow the samples to sit room temperature 10 – 15 minutes.

2. Before testing, mix contents of each vial by gently swirling. Be sure to re-suspend all red cells before proceeding.

3. Test each sample in the same manner as you would test a patient sample. If using a gel method, follow the gel card manufacturer’s instructions.

4. After sampling, replace the cap on each vial and return to the refrigerator.